ityhubhu ye-CPT 8ml

Inkcazelo emfutshane:

Umzekelo No.	CPT08
Izinto eziphathekayo	Iglasi yeGlasi engathathi hlangothi
Ukongeza	I-Anticoagulant + Gel + Ficoll Solution
Isicelo	Ukutsalwa kweeseli zeMononuclear kuphando
Ubungakanani bombhobho	16*120mm
Zoba uMqulu	8ML
Umgangatho wokhuseleko	Umgangatho woKhuseleko weHlabathi
Iiyunithi zokuthengisa	200 iityhubhu / ibhokisi
Umbala weCap	Mnyama & Bluwu
Isampulu	Iyafumaneka
Beka ubomi kwishelufa	Iminyaka eyi-1
OEM / ODM	Ileyibhile, izinto eziphathekayo, uyilo lwephakheji luyafumaneka.
Isiqinisekiso soMgangatho	ISO13485, FSC, MDSAP
Express	DHL, FedEx, TNT, UPS, EMS, SF, njl.
Intlawulo	L/C, T/T, Western Union, Paypal, njl.

Ukupakishwa

Iphakheji yomntu ngamnye kwisikhwama sephepha;

Thumela i-imeyile kuthi

Iinkcukacha zeMveliso

Iithegi zeMveliso

I-Vacutainer CPT (I-Cell Preparation Tube) I-8mL yityhubhu ekhethekileyo yokuqokelela igazi esetyenziselwa ukuhlukaniswa kweeseli ze-mononuclear (PBMCs) kwigazi elipheleleyo. Isetyenziswa ngokuqhelekileyo kwi-immunology, uphando lwezifo ezosulelayo, kunye nezicelo zeklinikhi ezifana nokujongwa komzimba kunye novavanyo olusekwe kwiseli.

Iimpawu eziphambili ze-Vacutainer CPT Tube (8mL):
Ukuzaliswa kwangaphambili nge-anticoagulant kunye ne-density gradient medium
Iqulethe i-Sodium Citrate njenge-anticoagulant.
Ibandakanya ijeli yokwahlula ingxinano ye-Ficoll™-based density gradient ukuququzelela ukwahlulwa kweeseli ze-mononuclear.
Yenza inyathelo elinye lokwahlulwa kwe-PBMC
Iphelisa imfuno yesiqhelo yoxinzelelo lwe-centrifugation yesandla (umz., Ficoll-Paque™).
Yehlisa ixesha lokucubungula kunye nokwahluka.
Umthamo wegazi osemgangathweni
Yenzelwe ukusebenza ukuya kuthi ga kwi-8mL yegazi elipheleleyo.
Ayinyumba, ityhubhu esetyenziswa kanye
Isetyenziselwa izicelo zeklinikhi kunye nophando apho ukuhlukaniswa kwe-PBMC ephezulu kuyadingeka.
Iyahambelana ne-centrifugation
Inkqubo ethile ye-centrifugation iyafuneka ukuze kwahlulwe ngempumelelo ii-PBMC kwezinye iincindi zegazi.

Ukusetyenziswa kunye nezicelo:
Ukwahlukaniswa kwe-PBMC kwizifundo zokusebenza komzimba (iiseli ze-T, iiseli ze-B, iiseli ze-NK, i-monocytes).
Flow cytometry, ELISPOT, cytokine assays, kunye nolunye uhlalutyo immunological.
Izifo ezosulelayo kunye nophando lwesitofu sokugonya.
Ukujongwa kwezifo ezizenzekelayo.
Izilingo zeklinikhi kunye nokufunyanwa kwe-biomarker.

Isebenza Njani (Inkqubo Esisiseko):
1. Ukuqokelela isampuli yegazi kwi-tube ye-CPT (gcwalisa kumthamo otyunjiweyo).
2. Guqula ngobumnene (i-8-10 amaxesha) ukudibanisa ne-anticoagulant.
3. I-Centrifuge ngokwe-protocol yomenzi (ngokuqhelekileyo malunga ne-1500-1800 RCF ngemizuzu engama-20-30 kwiqondo lokushisa).
4. Iifom zomaleko we-PBMC ngaphezu kwesahluli sejeli emva kokumiswa kwe-centrifugation.
5. Khupha ngononophelo umaleko we-PBMC usebenzisa i-pipette kwizicelo ezisezantsi.

Ukugcinwa kunye nokuphatha:
Gcina kwindawo yokushisa (15-25 ° C).
Kuphephe ubushushu obugqithisileyo okanye umkhenkce.